Effect of intracerebroventricular injection of leptin on proopiomelanocortin and neuropeptide Agouti related protein in type Ⅱ diabetic rats
10.3969/j.issn.1671-8348.2018.03.016
- VernacularTitle:侧脑室注射瘦素对2型糖尿病大鼠阿黑皮素原及神经肽Agouti相关蛋白的影响
- Author:
Ying DONG
1
;
Chunfeng LV
;
Wenhua LI
Author Information
1. 天津市北辰医院内分泌科 300400
- Keywords:
diabetes mellitus,type 2;
lateral ventricles;
leptin;
melanocortins;
neuropeptides
- From:
Chongqing Medicine
2018;47(3):337-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of intracerebroventricular injection of leptin on proopiomelanocortin(POMC) and neuropeptide Agouti related protein (AGRP) and fasting blood glucose (FBG) in rats with type 2 diabetes mellitus (T2DM).Methods Eighty male 8-week-age SD rats with T2DM were selected and divided into two groups:intracerebroventricular injection of leptin group(n=40)and intracerebroventricular injection of vehicle control group(n =40).Intracerebroventricular injection of 5 μL/kg(fasting leptin level 15 ng/mL) leptin was performed in the leptin injection group,and the control group was injected with same amount of vehicle instead.After 48 h,the FBG level was determined,and the levels of POMC and AGRP were detected by the titer method.Results The level of FBG in the leptin injection group was significantly lower than that in the control group(P＜ 0.05),the expression of serum POMC in the leptin injection group was significantly higher than that in control group(P＜0.05),The expression of AGRP in the leptin injection group was significantly decreased than control group(P＜0.05).Conclusion intracerebroventricular injection of leptin can significantly reduce the FBG level in rats with T2DM,increases POMC expression and decreases AGRP associated protein expression.
