Effects of tetrandrine on proliferation and activation of cardiac fibroblasts.
- Author:
Qian LI
1
;
Liang CHANG
2
;
Dong Mei SU
1
;
Xu MA
1
Author Information
1. Human Genetic Resources Center, National Research Institute for Family Planning, Beijing 100081, China.
2. Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.
- Publication Type:Journal Article
- MeSH:
Actins;
Animals;
Benzylisoquinolines/pharmacology*;
Blotting, Western;
Calcium Channel Blockers/pharmacology*;
Cell Proliferation;
Collagen;
Collagen Type I;
Fibroblasts/physiology*;
Fibrosis;
Myocardium/cytology*;
Neoplasm Proteins/metabolism*;
Rats;
Signal Transduction;
Transforming Growth Factor beta/metabolism*;
Transforming Growth Factor beta1
- From:
Journal of Peking University(Health Sciences)
2018;50(2):331-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of tetrandrine (Tet) on proliferation and activation of rat cardiac fibroblasts.
METHODS:Firstly, the cell counting kit-8 (cck-8) assay was applied to detect the effects of Tet with different concentrations on proliferation of cardiac fibroblasts. Secondly, transforming growth factor (TGF-β)with a concentration of 5 μg/L was used to induce the cardiac fibroblast activation, and Western blot was performed to measure the expression variation of β-catenin, vimentin (Vm), fibronectin (Fn) and smooth muscle α-actin (SMA). At last, the real-time PCR was conducted to measure the expression change of collagen-1(Col-1) and collagen-3(Col-3).
RESULTS:The cck-8 assay showed that the Tet with different concentrations respectively, which were 0.5 μmol/L, 1 μmol/L, 2 μmol/L, 4 μmol/L, and 8 μmol/L, significantly inhibited the proliferation of cardiac fibroblasts. The viability was decreased to 94.4%,84.9%,74.9%,63.8%and 50.3% respectively of the control group when the Tet concentration changed, and the difference was statistically significant, P=0.043, P<0.001, P<0.001, P<0.001, P<0.001 respectively. Western blot revealed that the expressions of β-catenin, Fn, SMA and Vm, were up-regulated by TGF-β(5 μg/L), the result showed that the difference was statistically significant, and the P values were 0.001,0.008,0.010,0.001 respectively. Then, the up-regulation of β-catenin, Fn and SMA was attenuated by pre-treatment of Tet, and the result also displayed that the difference was statistically significant, and the P values were 0.009, 0.005, 0.019,respectively. While there was no significant change in the expression of Vm, according to Western blotting, and P>0.05,at the same time, real-time PCR indicated that the up-regulations of Col-1 and Col-3 which were induced by TGF-β were blocked by pre-treatment of Tet, the result showed that the difference was statistically significant, P<0.001.
CONCLUSION:According to the experimental results, we can draw the conclusion that: the Tet can significantly inhibit the proliferation of cardiac fibroblasts, meanwhile, it can block the activation of cardiac fibroblasts, which is induced by TGF-β. It is supposed that the Tet may probably have anti myocardial fibrosis, which indicates that it may probably be a medicine which is used to block the cardiac remodeling.
