Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity.

Lucky Legbosi NWIDU; Raphael Ellis TEME

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Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity.

Author: Lucky Legbosi NWIDU ¹ ; Raphael Ellis TEME ²
Author Information

1. Department of Experimental Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Port Harcourt, Choba, Port Harcourt, PMB 5323 Rivers State, Nigeria. Electronic address: lucky.nwidu@uniport.edu.ng.
2. Histopathology Laboratory, Braithwaite Memorial Specialist Hospital, 50001 Rivers State, Nigeria; Medical Laboratory Science Department, Faculty of Science, Rivers State University of Science and Technology, Nkpoku Oroworukwo, Port Harcourt, PMB 5080 Rivers State, Nigeria.
Publication Type:Journal Article
Keywords: Hepatoprotection; Hepatotoxicity; Isoniazid; Lasianthera africana; Rats; Rifampicin; Tuberculosis
MeSH: Alanine Transaminase; metabolism; Animals; Antibiotics, Antitubercular; toxicity; Aspartate Aminotransferases; metabolism; Chemical and Drug Induced Liver Injury; drug therapy; etiology; metabolism; Cholesterol; metabolism; Female; Glutathione; metabolism; Humans; Isoniazid; toxicity; Liver; drug effects; enzymology; Magnoliopsida; chemistry; Male; Malondialdehyde; metabolism; Plant Extracts; administration & dosage; Plant Leaves; chemistry; Rats, Wistar; Rifampin; toxicity; Superoxide Dismutase; metabolism
From: Journal of Integrative Medicine 2018;16(4):263-272
CountryChina
Language:English
Abstract:
OBJECTIVESThe aim of this study is to evaluate the hepatoprotective effect of Lasianthera africana (Icacinaceae) against isoniazid (INH) and rifampicin (RIF)-induced liver damage in rats.
METHODSThe hepatoprotective effects of hot aqueous L. africana (HALA) leaf extract (0.1-1 g/kg) and silymarin (50 mg/kg) were assessed in a model of oxidative liver damage induced by RIF and INH (100 mg/kg each) in Wistar rats for 28 days. Biochemical markers of hepatic damage such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were assessed. The antioxidant statuses of plasma glutathione peroxidase (GSPx), glutathione reductase (GSH), catalase (CAT) and superoxide dismutase (SOD) and lipid peroxidation were evaluated.
RESULTSThe pretreatment of INH and RIF decreased hematological indices and the antioxidant levels (P < 0.001) and increased the levels of liver marker enzymes (P < 0.001). However, pretreatment with HALA extract and silymarin provoked significant elevation of hematological indices. The levels of AST, ALT, and ALP were depressed (P < 0.001). Total triglycerides, total cholesterol, total bilirubin and low-density lipoprotein were decreased (P < 0.001). However, high-density lipoprotein, bicarbonate, and electrolytes like chloride and potassium were elevated (P < 0.001), but sodium was depressed (P < 0.05). Additionally, GSH, GSPx, SOD and CAT were elevated (P < 0.01) and malondialdehyde was depressed (P < 0.001) when compared to the RIF-INH-treated rats. Histopathological evaluations support hepatoprotective activity.
CONCLUSIONThis study demonstrated that HALA leaf extract attenuated RIF-INH-induced hepatotoxicity. L. africana could be exploited in management of RIF-INH-induced hepatitis.