OBJECTIVETo investigate a modified protocol of establishing mouse Ph ALL model so as to provide a more convenient and more powerful tool for Ph ALL studies.

METHODSImmature B cells from BALB/c mice were transfected with the Mig190 retrovirus and infused into irradiated syngeneic mice. The immunophenotype was identified by flow cytometry, the BCR-ABL was identified by RT-PCR and Western blot. Leukemia cells isolated from sick mice were re-infused into syngeneic mice without irradiation.

RESULTSThe acute lymphoblastic leukemia was established in mice after Mig190 retroviral transfection, the lymphoblasts were observed by blood smears, the immunophenotype of these leukemic cells was CD19, and the BCR-ABL was detected by RT-PCR and Western blot, the immunophenotype was conformed as Ph ALL, the isolated leukemia cells infused into mice rapidly developed B-ALL.

CONCLUSIONA new protocol is established to generate mice with Ph ALL. This mouse model can provide a more simple and effective tool in comparison with the conversional protocols.