Inhibitory effect of Scutellariae Radix on hepatic fibrosis based on urinary metabonomic.
10.19540/j.cnki.cjcmm.20180208.002
- Author:
Hong CHANG
1
;
Hong-Yu MENG
2
;
Yu WANG
1
;
Zhan TENG
1
;
Shu-Min LIU
1
Author Information
1. Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
2. Dongzhimen Hospital, Beijing University of Chinese Medcine, Beijing 100010, China.
- Publication Type:Journal Article
- Keywords:
Scutellariae Radix decoction;
baicalin;
hepatic fibrosis;
urinary metabonomics
- From:
China Journal of Chinese Materia Medica
2018;43(10):2140-2146
- CountryChina
- Language:Chinese
-
Abstract:
Urinary metabolomics combined with histological progression were utilized to evaluate the therapeutic effect of Scutellariae Radix decoction and baicalin on hepatic fibrosis (HF) and explore their mechanisms, intervention targets and metabolic pathways. HF rat model was established through subcutaneous injection of CCl₄ for 8 weeks. Meanwhile, different doses of Scutellariae Radix decoction and baicalin were administered. Histomorphology of liver tissue was observed and scored by HE and Masson. Urinary metabonomic analysis based on UPLC-Q-TOF-MS was made for the changes of urinary potential biomarkers among different groups at different time points of HF. Finally, it was found that Scutellariae Radix decoction could improve HF by regulating L-tryptophan, 3-methyldioxyindole, 5-hydroxyindoleacetylglycine, kynurenic acid, 4-(2-amino-3-hydroxyphenyl)-2,4-dioxobutanoic acid, methylmalonic acid and L-leucine. However, baicalin could improve HF by regulating L-tryptophan, 3-methyldioxyindole, 5-hydroxyindoleacetylglycine, 4-(2-amino-3-hydroxyphenyl)-2,4-dioxobutanoic acid, kynurenic acid, and methylmalonic acid. These metabolites involved in tryptophan metabolism and valine, leucine and isoleucine degradation pathways. These results indicated that Scutellariae Radix had the multi-target and multi-pathway characteristics in the treatment of HF. Additionally, low-dose Scutellariae Radix decoction and baicalin are showed better efficacies, with no statistically significant difference between them in histomorphology.
