Determination of flavonoids constituents in epimedium total flavone capsule and research on its anti-osteoporosis activity.
- Author:
Zhong-Kun XU
1
;
Hong-Mei YIN
1
;
Fang LI
1
;
Jian-Ping QIN
1
;
Shao-Li GU
1
;
Yun WU
1
;
Zhen-Zhong WANG
1
;
Wei XIAO
1
Author Information
- Publication Type:Journal Article
- Keywords: AKP; MC3T3-E1; RRLC; epimedium total flavone capsule; flavonoid glycoside
- From: China Journal of Chinese Materia Medica 2018;43(15):3140-3144
- CountryChina
- Language:Chinese
- Abstract: To develop a rapid resolution liquid chromatography (RRLC) method for the simultaneous determination of epimedoside A, epimedin A1, epimedin A, epimedin B, epimedin C, icariin, baohuosideⅡ, icarisideⅠ, sagittatoside B, 2"--rhamnosyl icarisideⅡ, and baohuosideⅠin epimedium total flavone capsule. At the same time, the effects of the above 11 compounds on osteogenic differentiation of MC3T3-E1 cells were investigated by detecting the content of alkaline phosphatase (AKP). The results showed that baohuoside Ⅱ had the highest activities, and both the activities of baohuoside Ⅱ and icariside Ⅰ were stronger than those of icariin.In this study, the content determination method of flavonoid glycosides was established, and the anti-osteoporosis effect of monomers was compared, providing technical support for the study of the pharmacodynamic and mechanism of Epimedium total flavone capsule.
