Galectin3 Regulates Transforming Growth Factor-β-induced Epithelial-mesenchymal.
10.3881/j.issn.1000-503X.2018.02.012
- VernacularTitle:半乳糖凝集素3调控转化生长因子-β诱导的A549细胞上皮间质转化
- Author:
Yong Qiang WANG
1
;
Zi Hou LIU
1
;
Jie SHU
2
;
Shen E TANG
2
;
Fan Yan LUO
1
Author Information
1. Department of Cardiac Surgery, Central South University,Changsha 410008,China.
2. Institute of Medical Sciences,Xiangya Hospital,Central South University,Changsha 410008,China.
- Publication Type:Journal Article
- From:
Acta Academiae Medicinae Sinicae
2018;40(2):219-224
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of Galectin3 in transforming growth factor-β(TGF-β)-induced epithelial-mesenchymal transition (EMT) in A549 cells. Methods Galectin3 was over-expressed in an A549 cell line. EMT was induced in lung cancer A549 cells by adding TGF-β. The expressions of Galectin3,E-cadherin,and vimentin were determined by Western blot. The protein expression of E-cadherin and the morphological changes of the cells were detected by immunofluorescence. Cellular proliferation was analyzed with cell counting kit-8,and the cellular migration and invasion was measured by scratches healing and Transwell assay,respectively.Results When only Galectin3 was over-expressed in A549 cell line,the expression levels of EMT-related proteins such as E-cadherin and vimentin were not changed,and the abilities of cellular proliferation,invasion,and migration were not changed either. When the EMT was induced by TGF-β in A549 cells,the E-cadherin expression was down-regulated and the vimentin expression was up-regulated in A549 cells with Galectin3 over-expression. There was no significant change in cellular proliferation,whereas the abilities of cellular invasion and migration were enhanced.Conclusion The TGF-β-induced EMT in A549 cells can be enhanced by Galectin3.
