Regulatory effect of Shudihuang on expressions of BDNF/TrkB and NRG-3 in prefrontal cortex and striatum of ADHD model rats.
10.19540/j.cnki.cjcmm.20180522.004
- Author:
Hai-Xia YUAN
1
;
Xin-Qiang NI
2
;
Zheng-Zhi WU
3
;
Xin-Min HAN
1
;
Dan-Gui XU
2
;
Yu-Chen SONG
1
;
Tian-Yi CHEN
1
Author Information
1. Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China.
2. Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518038, China.
3. Shenzhen Institute of Geriatrics, Shenzhen 518035, China.
- Publication Type:Journal Article
- Keywords:
SHR;
Shudihuang;
attention deficit hyperactivity disorder(ADHD);
brain-derived neurotrophic factor;
neuregulin-3;
tyrosine protein kinase B
- From:
China Journal of Chinese Materia Medica
2018;43(17):3539-3544
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effect of Shudihuang on behaviors and expression of BDNF/TrkB and NRG-3 in prefrontal cortex and striatum of attention deficit hyperactivity disorder (ADHD) model rats. Thirty 4-week-old spontaneous hypertension rats (SHR) were randomly divided into model group, methylphenidate hydrochloride (MPH, 2 mg·kg⁻¹·d⁻¹) and Shudihuang group (2.4 g·kg⁻¹·d⁻¹). Another 10 Wistar-Kyoto (WKY) rats were selected as normal control group. The 0.5% CMC-Na solution was administered to model group and WKY rats (2 mL·kg⁻¹·d⁻¹). All of the rats were treated for 4 weeks. The open field test was performed at the 14th and 28th days after gavage, in order to evaluate the spontaneous and impulsive behaviors. Subsequently, gene and protein expressions of BDNF/TrkB and NRG-3 were tested by RT-qPCR and Western blot. Compared with model group, MPH and Shudihuang groups showed significant reduction in total distance, mean velocity and central distance in the open field test (<0.05), and Shudihuang group displayed a shorter central distance than MPH group (<0.05). RT-qPCR and Western blot analysis indicated that expressions of BDNF/TrkB and NRG-3 were lower in prefrontal cortex and striatum of SHR compared with WKY rats. Four weeks later after administration, both Shudihuang and MPH significantly elevated mRNA and protein expressions of BDNF/TrkB and NRG-3 (<0.05).In conclusion, neurodevelopmental disorder mediated by BDNF/TrkB and NRG-3 was closely related with SHR rats' behaviors. Shudihuang may ameliorate the spontaneous and impulsive behaviors by up-regulating the expressions of BDNF/TrkB and NRG-3 and improving growth and maturation of neurons in SHR.
