Pharmacokinetics of eight constituents in rat plasma after oral administration of Modified Xiaochaihu Granules for gastric ulcer based on UPLC-MS/MS.
10.19540/j.cnki.cjcmm.2018.0104
- Author:
Meng-Ling YANG
1
;
Peng-Tao YOU
1
;
Li-Shan HE
2
;
Shu-He CHEN
3
;
Ai-Jun ZHOU
4
;
Yan-Wen LIU
1
;
Xin CHEN
1
Author Information
1. Hubei Provincial Key Laboratory for Resource and Chemistry of Chinese Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China.
2. Wuhan Institute for Food and Cometic Control, Wuhan 430012, China.
3. Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430060, China.
4. Dongguan Hospital of Traditional Chinese Medicine, Dongguan 523000, China.
- Publication Type:Journal Article
- Keywords:
Modified Xiaochaihu Granules;
gastric ulcer;
pharmacokinetics
- From:
China Journal of Chinese Materia Medica
2018;43(18):3748-3755
- CountryChina
- Language:Chinese
-
Abstract:
An ultra-performance liquid chromatography-tandem mass spectrometric method (UPLC-MS/MS) was developed in this study to simultaneously determine the contents of eight effective constituents in rat plasma, including baicalin, wogonoside, baicalein, liquiritin, glycyrrhizic acid, berberine hydrochloride, saikosaponin a and saikosaponin d in plasma of gastric ulcer rats, and investigate the pharmacokinetics of Modified Xiaochaihu Granules. Chromatographic separation was conducted on Zorbax SB-C₁₈ column (2.1 mm×100 mm, 1.8 μm) with acetonitrile -0.1% formic acid aqueous solution as the mobile phase for gradient elution, at a flow rate of 0.4 mL·min⁻¹ and column temperature of 40 °C. Detection was performed in the multiple reaction monitoring (MRM) mode with ESI ion source. All calibration curves showed good linearity (>0.996) over a wide concentration range for all constituents. RSDs of intra-day and inter-day precision were all within 15% and the extraction recoveries of all the constituents were in the range of 81.92% to 104.8%. The time to peak (tmax) of these eight constituents was (2.69±2.02), (5.17±2.04), (0.25±0), (0.83±0.26), (0.92±0.20), (0.92±0.20), (0.58±0.20), and (0.083±0) h, respectively; the half-life (t1/2) of them was (7.85±0.34), (10.16±2.21), (6.79±0.21), (8.32±0.48), (11.05±1.78), (11.56±3.46), (15.30±1.84), and (5.54±1.91) h, respectively; the peak concentration (Cmax) of them was (55.02±1.67), (213.66±4.62), (62.61±0.69), (68.43±1.42), (62.22±0.39), (30.17±1.89), (61.79±4.81), and (38.02±1.75) μg·L⁻¹, respectively. This established method is simple and accurate with good repeatability and strong specificity, which could provide modern experimental basis for modified Xiaochaihu granules in clinical treatment of gastric ulcer.
