BRAF-V600E mutation and its clinical significance in children with Langerhans cell histiocytosis.
- Author:
Xue TANG
1
,
2
;
Xia GUO
;
Lin-Yong SUN
;
Yuan AI
;
Xue YANG
;
Jing-Jing SUN
;
Jian-Rong WU
;
Ju GAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; Child, Preschool; Female; Histiocytosis, Langerhans-Cell; classification; genetics; mortality; Humans; Infant; Male; Mutation; Proto-Oncogene Proteins B-raf; genetics; Retrospective Studies
- From: Chinese Journal of Contemporary Pediatrics 2018;20(4):290-294
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the clinical significance of BRAF-V600E mutation in children with Langerhans cell histiocytosis (LCH).
METHODSReal-time fluorescence quantitative PCR was used to detect BRAF-V600E mutation in paraffin-embedded tissue samples from 26 children with LCH. A retrospective analysis was performed for the association of BRAF-V600E mutation with clinical features and prognosis of children with LCH.
RESULTSOf the 26 children, 25 received standard chemotherapy, with a 2-year overall survival (OS) rate of 100% and a 2-year event-free survival (EFS) rate of 88%. Of the 26 pathological samples, 18 (70%) came from bone tissue, and the positive rate of BRAF-V600E mutation reached 50% (13/26). The positive rate of BRAF-V600E gene mutation was not associated with age, sex, affected organ, clinical classification, early treatment response, recurrence, and 2-year OS and EFS rates of the children with LCH (P>0.05), but it was associated with clinical grouping of LCH (P<0.05).
CONCLUSIONSChildren with LCH tend to have a high OS rate and a high incidence rate of BRAF-V600E mutation. BRAF-V600E mutation is associated with clinical grouping of LCH.
