Effects of Coix-seed Reactive Derivatives (CRD) on Cytochrome P450 Isoforms CYP3A4, CYP2D6, CYP2C9 In Vitro

VernacularTitle:Coix-seed Reactive Derivatives (CRD)の薬物代謝酵素CYP3A4，CYP2D6，CYP2C9に与える影響
Author: Nobutaka SUZUKI ¹ ; Hoko KYO ¹ ; Kazuo UEBABA ² ; Tomihisa OHTA ³
Author Information

1. Department of Complementary and Alternative Medicine Clinical Research and Development, Kanazawa University Graduate School of Medical Science
2. Teikyo Heisei University
3. Advanced Science Research Center, Kanazawa University
Keywords: Coix lacryma-jobi L. var. ma-yuen Stapf; Coix Reactive Derivatives; CYP3A4; CYP2D6; CYP2C9
From:Japanese Journal of Complementary and Alternative Medicine 2018;15(2):109-112
CountryJapan
Language:Japanese
Abstract: CRD, a Coix-seed Reactive Derivatives, has a novel mechanism in the treatment of various diseases. In the present study, a fluorometric-based high throughput method using cytochrome P450 (CYP)screening kit was adopted to evaluate in vitro inhibition potential of CRD (CRD 1 and CRD 2) on CYP isoenzymes by calculating remaining enzyme activities and inhibitory potential (IC50 values) using the determined values of fluorescence intensity. IC50　of CRD 1 and CRD 2 were as follows: CYP3A4; >500 µg/ml, 490 µg/ml, CYP2D6; >500 µg/ml，>500 µg/ml，CYP2C9; >500 µg/ml，339 µg/ml, respectively. The result showed that CRD exhibited little activity in the inhibition of CYP3A4, CYP2D6 and CYP2C9.