Preparation process of norcantharidin/tetrandrine dual loaded liposomes and their release characteristics.
10.19540/j.cnki.cjcmm.20180514.007
- Author:
You-Xiang XIONG
1
;
Hong-Xia TANG
1
;
Rui MA
1
;
Fan-Zhu LI
1
Author Information
1. School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 311400, China.
- Publication Type:Journal Article
- Keywords:
double loaded liposomes;
encapsulation efficiency;
in vitro release;
norcantharidin;
tetrandrine
- From:
China Journal of Chinese Materia Medica
2018;43(12):2531-2536
- CountryChina
- Language:Chinese
-
Abstract:
In order to optimize the prescription and preparation process of norcantharidin/tetrandrine dual loaded liposomes, the dual drug loaded liposomes were prepared by film dispersion-ultrasonic method using norcantharidin-mesoporous silica nanoparticles（MSN-NCTD）and tetrandrine（Tet）. With particle size and encapsulation efficiency as comprehensive indexes, the influences of phospholipid cholesterol amount, ultrasonic time and ultrasonic power on prescription process were investigated by orthogonal test; the release characteristics of liposomes were investigated by dialysis method. The results indicated that the best prescription process of prepared norcantharidin/tetrandrine dual loaded liposomes was as follows: phospholipid-cholesterol ratio 2.5:1, ultrasonic time 4 min, ultrasonic power 40%; the encapsulation efficiency was 86.62% and 79.19%respectively for NCTD and Tet;liposomes were well-shaped under the transmission microscope, with average particle size of （207.5±3.6） nm, Zeta potential of （1.345±0.173） mV; and the 48 h cumulative release rates of NCTD and Tet were 85.14% and 85.00% respectively. The experiment results proved that the dual drug loaded liposomes prepared by film dispersion-ultrasonic method had uniform particle size, high encapsulation efficiency and sustained release characteristics.
