Synthesis and identification of artificial antigen of spinosin.
10.19540/j.cnki.cjcmm.20180404.001
- Author:
Biao ZHANG
1
;
Yan-Chuan LI
2
;
Hong FENG
2
;
Hong-Liang XU
2
;
Lan-Lan ZHANG
2
;
Ping LI
1
Author Information
1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
2. Digital Materia Medica Traditional Chinese Medicine Testing Co., Ltd., Baoding 071200, China.
- Publication Type:Journal Article
- Keywords:
antibody;
artificial antigen;
immunoassay method;
sodium periodate oxidation method;
spinosin
- From:
China Journal of Chinese Materia Medica
2018;43(13):2747-2750
- CountryChina
- Language:Chinese
-
Abstract:
Immunogenic antigen (spinosin-BSA) and coating antigen (spinosin-OVA) of spinosin were synthesized by sodium periodate oxidation method. UV scanning analysis method showed that these two spinosins were successfully conjugated with carrier protein and the coupling ratio was 17 and 13.7, respectively. Meanwhile, when immunized by spinosin-BSA，the mice can produce anti-spinosin antibodies with the high titer (1:32 000),specificity (IC₅₀ 211.6 μg·L⁻¹) and low cross-reaction rate measured by ELISA tests. The artificial antigen of spinosin was successfully synthesized, which can be applied for preparation of monoclonal antibodies and establishment of appropriate immune method.
