Protective effect of Longxue Tongluo capsule on oxidized low-density lipoprotein damage to human umbilical vein endothelial cells.
10.19540/j.cnki.cjcmm.20180104.023
- Author:
Hong-Mei WANG
1
;
Jian-Ming ZHOU
1
;
Yao-Zhong LV
1
;
Jun ZHOU
1
;
Zhen-Zhong WANG
1
;
Wen-Zhe HUANG
1
;
Peng-Fei TU
2
;
Wei XIAO
1
Author Information
1. Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China.
2. School of Pharmacy, Peking University, Beijing 100083, China.
- Publication Type:Journal Article
- Keywords:
ICAM-1;
IKB;
LDH;
Longxue Tongluo capsule;
MDA;
NO;
SOD;
VCAM-1;
human umbilical vein endothelial cells;
ox-LDL;
p-IKB;
p-p65;
p65
- From:
China Journal of Chinese Materia Medica
2018;43(6):1241-1246
- CountryChina
- Language:Chinese
-
Abstract:
To observe the protective effect of Longxue Tongluo capsule (LTC) on human umbilical vein endothelial cells (EAhy.926 cells) injury induced by oxidized low-density lipoprotein (ox-LDL, 100 mg·L⁻¹). The effect of the cell viability of LTCin alleviating OX-LDL-induced endothelial cell injury was determined by MTT and LDH assay. The effect of LTC on lactic dehydrogenase (LDH), nitric oxide (NO), super oxide dlsmutase (SOD) and malondialdehyde (MDA) levels were detected by corresponding assay kits according to manufacturer's instruction. The effect of LTC on the protein expressions of intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), p65, p-p65, IKB and p-IKB were detected by Western blot. The results showed that compared with the normal control group, the activity of EAhy.926 cells was significantly decreased, LDH leakage (<0.01) increased, NO content and SOD activity significantly decreased (<0.01, <0.05), and the expressions of ICAM-1, VCAM-1, p-p65/p65 and p-IKB(<0.05)increased.This study demonstrated that LTC had no significant effect on the growth of normal cells. The treatment with LTC significantly promoted the proliferation of vascular endothelial cells damagedby ox-LDL, decreased MDA content and LDH release, andincreased the activity of SOD and NO content. Meanwhile, ox-LDL significantly increased the expressions of ICAM-1, VCAM-1, p-p65/p65, p-IKB/IKB in Eahy.926 cells; these effects were suppressed by LTC at 1, 2 mg·L⁻¹. In conclusion, LTC has a significant protective effect on human umbilical vein endothelial cells caused by ox-LDL. This study suggested that LTC has a certain therapeutic effect on AS.
