Highly sensitive assay for the determination of therapeutic peptide desmopressin in human plasma by UPLC–MS/MS
- Author:
Gudlawar Kumar Shiva
;
Pilli Rao Nageswara
;
Siddiraju Sridhar
;
Dwivedi Jaya
- Keywords:
Desmopressin;
Solid-phase extraction (SPE);
Ultra performance liquid chromatography–tandem mass spectrometry;
Method validation;
Pharmacokinetics
- From:
Journal of Pharmaceutical Analysis
2017;7(3):196-202
- CountryChina
- Language:Chinese
-
Abstract:
An analytical method based on ultra-performance liquid chromatography with positive ion electrospray ionization (ESI) coupled with tandem mass spectrometry (UPLC–MS/MS) was developed and validated for the determination of therapeutic peptide desmopressin in human plasma. A desmopressin stable labeled isotope (desmopressin d8) was used as an internal standard. Analyte and the internal standard were extracted from 200 μL of human plasma via solid-phase extraction technique using Oasis WCX cartridges. The chromatographic separation was achieved on an Aquity UPLC HSS T3 column by using a gradient mixture of methanol and 1 mM ammonium formate buffer as the mobile phase. The calibration curve obtained was linear (r2≥0.99) over the concentration range of 1.01–200 pg/mL. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. The proposed method was successfully applied to pharmacokinetic studies in humans.
