Serum-free Aggregate Perfusion Culture of CHO Cells:an Ultrasonic and Sedimentation Column Combined Perfusion System
- VernacularTitle:CHO细胞无血清结团灌流培养:超声-沉降柱二合一灌流系统
- Author:
Zhi LI
;
Cheng-Zu XIAO
;
Qin YANG
;
Xiao-Le HUANG
;
Qian-Ru LIANG
;
Xiao-Fei CHEN
;
Dun-Wu ZHENG
;
Xiao-Ming CHEN
;
- Publication Type:Journal Article
- Keywords:
Aggregation culture Serum-free media CHO cells TNK-tPA Ultrasonic and sedimentation column combined perfusion system
- From:
China Biotechnology
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Using the character of natural aggregation of CHO cells, and an ultrasonic and sedimentation column combined perfusion system to promote cells aggregation and retention into bioreactor,recombinant CHO cell strain MK3-A2 was cultured,which could secrete rhTNK-tPA, by a serum-free perfusion culture system. The culture periods in this two experiments were as long as 77 and 110 days respectively. The cells density reached 2?107 cells /ml. The average volumetric productivity of rhTNK-tPA was 89 mg/L?d, and the highest one was 216mg/L?d.The cells aggregation rate was approximately 90%, and the diameters of most of them were 285～570?m. During the perfusion culture the cells retention rate almost kept in 95% and the viability of cells was more than 85%.Thus, it means that aggregation culture with such perfusion system could be used to scale up produce biopharmaceuticals instead of microcarrier culture system.
