Effects of pinacidil on intracellular free calcium concentration of cultured neonatal rat cardiomyocytes during hypoxia/reoxygenation
- VernacularTitle:吡那地尔对低氧/复氧乳鼠心肌细胞内游离钙浓度的影响
- Author:
Hexiang CHENG
;
Rongqing ZHANG
;
Guoliang GIA
;
Xinguo ZHAO
;
Bing LIU
;
Dan CHEN
;
- Publication Type:Journal Article
- Keywords:
Myocardium;
Hypoxia/reoxygenation;
Calcium;
Pinacidil
- From:
Journal of Medical Postgraduates
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To examine effects of pinacidil on intracellular free calcium concentration of cardiomyocytes during hypoxia/reoxygenation. Methods:A cell culture model of neonatal rat cardiac myocytes was used. There were three groups, including control group, hypoxia/reoxygenation group and pinacidil group. Confocal microscope was used with Fluo 3/AM as calcium indicator to detect changes of intracellular free calcium concentration. Results:The intracellular fluoresence intensity of singular cardiomyocyte in hypoxia/reoxygenation group was significantly higher than that of the controls( P
