Effect of chloride channel blockers on volume-sensitive chloride channels in mouse cardiac ventricular myocytes
- VernacularTitle:氯通道阻断药对小鼠心室肌容积敏感性氯通道的影响
- Author:
Hongtao XU
;
Qiyu GUO
;
Lianru GAO
;
Yuxing FEI
;
Zhiming ZHU
;
- Publication Type:Journal Article
- Keywords:
Patch clamp;
Volume sensitive chloride channel;
Ventricular myocytes;
NPPB;
Tamoxifen;
Mouse
- From:
Journal of Medical Postgraduates
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To examine the effect of chloride blocker (NPPB and tamoxifen)on volume sensitive chloride channels in mouse cardiac ventricular myocytes. Methods:Isolated mouse cardiac ventricular myocytes were subjected to whole cell patch clamp to record the hypotonicity activated chloride currents. Results:When the myocytes were exposed to hypotonic solution, an obvious whole cell currents were activated. The currents were inhibited by extracellular NPPB reversibly and significantly. The specific blocker for volume sensitive chloride channel , tamoxifen (50 ?mol/L), could apparently block the activity of this channel in a voltage dependent manner. Conclusions:Mouse cardiac ventricular myocytes process volume sensitive chloride channel which is sensitive to NPPB and tamoxifen.
