Immunostimulatory Activity Elicited by CpG Sequences in Cysticercus Cellulosae Paramyosin cDNA
- VernacularTitle:猪囊尾蚴副肌球蛋白cDNA中CpG序列的免疫激活作用
- Author:
Shuhan SUN
;
Yingjun GUO
;
Qingmin WANG
;
Ruiwen CHEN
;
- Publication Type:Journal Article
- Keywords:
Cysticercus cellulosae,paramyosin,DNA vaccine,CpG sequence
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the immunostimulatory activity of CpG sequences in cysticercus cellulosae paramyosin (also named Antigen B,AgB)cDNA. Methods C57BL/6 mice were immunized with pcDNA3 AgB plasmid,pcDNA3 AgB′(CpG sequences were mutated),pcDNA3 or AgB protein and two weeks later,immune response was assayed by ELISA. Results IgG and IgG 2a were detectable at week 2 after immunization and continually increased until week 4.The antibody levels elicited by pcDNA3 AgB were significantly higher( P
