BREEDING OF A HIGH-?-LINOLENIC ACID YIELD PRODUCING STRAIN AND THE EXTRACTION OF FERMENTATION PRODUCT
- VernacularTitle:?-亚麻酸高产菌株的选育及发酵产物的分离提取
- Author:
Jun ZHANG
;
Laijun XING
;
Hongmei WANG
;
- Publication Type:Journal Article
- Keywords:
?-Linolenic acid (GLA);
Mortierella;
Fermentation
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Mortieralla isbellina (AS 3.3410) was used as the starting strain in the experiment. Through fermentation, the production rate of fungal body was 10%, content of lipid in fungal body was 27%, content of ?-Linolenic acid in lipid was 3.3%, When it was treated by U.V, mutant M_6 was obtained, its production rate of fungai body was 25%, lipid content was 32.8%, ?-linolenic acid content was 8.44%. Pass-generation test indicated that the heredity character of M_6 was stable.The optimum culture medium was obtained through culture condition test, and the result of fermentation in 10L tank showed that the production rate of fungal body was 29.3%, lipid content was 44.7%, GLA content was 9.44%.In the meantime, the method of extraction of fungai lipid was primarily studied, which indicated. that it is preferable to use a combination of ethanol and hexane to extract alternately.
