Protective effect of treatment of somatostatin combined with growth hormone on brain injury in severe acute pancreatitis rats
- VernacularTitle:生长抑素联合生长激素治疗对重症急性胰腺炎大鼠脑损伤的保护作用
- Author:
Baobing YIN
;
Baojin MA
;
Duan CAI
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
Somatostatin;
Somatotropin;
Brain damage
- From:
Chinese Journal of Digestion
1996;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of treatment of somatostatin combined with growth hormone on brain injury in early severe acute pancreatitis(SAP)rats, and to investigate the relationship between brain injury and ratio of endothelin 1(ET 1) /nitric oxide(NO). Methods SAP model was established by retrograde pancreatic bile duct injection of 3.5% sodium taurocholate at a dose of 2.5 ml/kg in rats. Eighty SAP rats were equally divided into SAP group, somatostatin (S) group (introvenous injection of somatostatin at a dose of 42 ?g/kg once a day for 2 days), somatotropin (G) group (subcutaneous injection of somatotropin at a dose of 0.5 ?g/kg once a day for 2 days) and S+G group. Twenty normal rats were used as controls. The changes of encephaledema and blood brain barrier permeability were measured by dry wet method and Evan′s blue staining, respectively. Apoptosis of brain cells was detected by TUNEL method, and the ratio of serum ET 1/NO was also determined. Results The ratio of serum ET 1/NO was remarkably increased in SAP rats, which was correlated with the intensity of brain edema, permeability of blood brain barrier and apoptosis of brain cells. The treatment of somatostatin combined with growth hormone reduced the ratio of ET 1/NO and thus decreased the intensity of encephaledema, the permeability of blood brain barrier and apoptosis of brain cells. The changes of the brain slow wave were found simultaneously by electroencephalography in SAP rats after the therapy. Conclusions The treatment of somatostatin combined with growth hormone in SAP rats can inhibit serum ET 1/NO, prevent the development of brain edema, decrease the permeability of blood brain barrier, and alleviate the brain cells apoptosis. All these result in the relief of the brain injury.
