Determination of rehmannioside D in root ofRehmannia glutinosa by HPLC
- VernacularTitle:HPLC法测定地黄中地黄苷D含量
- Author:
Gengsheng LI
;
Huisen WANG
;
- Publication Type:Journal Article
- Keywords:
the root of Rehmannia glulinosa (Gaertn.) Libosch. ex Fisch et Mey;
rehmannioside D;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To establish an HPLC method for the determination of rehmannioside D in the root of Rehmanniae glutinosa (Gaert.) Libosch. ex Fisch et Mey and provide a limitation for the lowest content of rehmannioside D Methods Chromatographic conditions: Hypersil C 18 (200 mm?4.6 mm, 5 ?m) column, mobile phase: acetonitrile water (4∶96), flow rate: 1 mL/min, room temperature, detection wavelength: 205 nm Results The recovery was 100.6%, and RSD was 2.1% for rehmannioside D. From eight different regions 16 samples of R glutinosa were quantitatively analysed and the contents of rehmannioside D in R glutinosa were 0.137%-0.691% Conclusion The method is rapid, simple and accurate, and can be used for the quality control of R glutinosa The proposed content of rehmannioside D must be no less than 0.15%.
