EXPRESSION OF ABC GENE FAMILY IN MCF-7/ADM CELL LINE AND THE REVERSAL EFFECT OF REALGAR ON THESE GENES
- VernacularTitle:ABC家族基因在乳腺癌耐药细胞MCF-7/ADM中的表达及雄黄对其作用初探
- Author:
Yuhua WANG
;
Changhui GE
;
Jinyao ZHAO
;
Peiman YANG
;
- Publication Type:Journal Article
- Keywords:
ABC gene family;
MDR;
Realgar;
Breast cancer
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of ABC gene family of MCF 7/ADM cells as compared to parental cell line MCF 7/S, and its reversion by realgar of the expressed genes. Methods Expression of ABC family genes was examined by RT PCR, and cell growth by MTT test. Results The expression of ABC gene family was positive in MCF 7/ADM cells compared to MCF 7/S cells; Realgar can down regulate the expression ABC gene family in MCF 7/ADM cells; the IC 50 of ADM to MCF 7/ADM cells was decreased.Conclusion ABC gene family was responsible for the induced resistance of MCF 7/ADM cells to ADM; Realgar is able to reverse partly the multidrug resistance of the human breast cancer cell line MCF 7/ADM. [
