Determination of cinnamic acid in Lingguizhugan Decoction by RP-HPLC
- VernacularTitle:RP-HPLC测定苓桂术甘方中肉桂酸的含量
- Author:
Xiaohong HUANG
;
Zonghua SONG
;
Xiuqin LI
;
Kaishun BI
;
- Publication Type:Journal Article
- Keywords:
RP HPLC;
Lingguizhugan Decoction;
cinnamic acid
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the determination of cinnamic acid in Lingguizhugan Decoction (Poria, Ramuls Cinnamomi, Rhizoma Atractylodis macrocephalae, Radix Glycrrhizae, etc.). Methods: Using RP HPLC with Hypersil C 18 column, methanol acetonitrile water acetic acid (10∶22∶70∶1.4,v/v) as mobile phase, detection wavelength at 254nm and p dimethylaminobenzaldehyde as the internal standard.Results: The mean recovery and RSD were 98.2% and 1.9% ( n =6), respectively, the linear range of cinnamic acid was 8.4～168?g?mL -1 ( r =0.9999). Conclusion: The method is simple, rapid, accurate and reproducible, and may be used for the determination of cinnamic acid in Lingguizhugan Decoction.
