STUDY ON APOPTOSIS IN ACUTE ISCHEMIC AND REPERFUSED RAT MYOCARDIUM
- VernacularTitle:大鼠急性心肌缺血再灌注的心肌细胞凋亡研究
- Author:
Ming XU
;
Qingyun ZENG
;
Chengying DING
;
- Publication Type:Journal Article
- Keywords:
Myocardium;
Apoptosis;
Ischemia/Reperfusion;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To demonstrate in vivo that there is different degree myocardium apoptosis during acute ischemia and reperfusion and to explore prelimarily the relations between myocardium apoptosis and the expression of Bcl 2/Bax protein in rats. Methods The morphologic features of apoptotic cardiocytes were pursuited by transmission electron microscopy.DNA extracted from cardiocytes were used to identify the apoptotic DNA ladder by agarose gel eletrophoresis. DNA fragmentation was identified histochemically by TUNEL staining. Expression of Bcl 2/Bax protein in apoptotic myocardium was observed by means of immunohistochemistry and analysed by image analysis system. Results In both ischemia and reperfusion groups morphologic features of apoptotic myocardium were observed by transmission electron microscopic and the apoptotic cardiocytes were detected by the TdT mediated dUTP nick end labeling (TUNEL) staining. However, the apoptotic DNA ladder was observed only in the 2 hour reperfusion group. The reperfusion group showed a decrease in OD value of the Bcl 2 protein( P
