Effects of lentinan on nitric oxide production and intracellular glutathione in mouse peritoneal macrophages
- VernacularTitle:香菇多糖对巨噬细胞一氧化氮生成和细胞内还原型谷胱甘肽的影响
- Author:
Gan HOU
;
Dinan HUANG
;
Qifeng ZHU
;
- Publication Type:Journal Article
- Keywords:
lentinan (LTN);
macrophages;
nitric oxide (NO);
glutathione (GSH)
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To investigate the mechanisms of action of lentinan (LTN) activated mouse peritoneal macrophages to produce nitric oxide (NO). Methods The effects of LTN on NO output and intracellular glutathione (GSH) in mouse peritoneal macrophages and the correlation of them were studied. Results (1) LTN can significantly increase production of NO in mouse peritoneal macrophages, decrease level of intracellular GSH followed increase of NO production. (2) The above effect can be blocked efficiently by the NO production inhibitors. (3) NO production can be inhibited by GSH lowering drugs. Conclusion LTN increase NO production with depletion of intracellular GSH in the activated mouse peritoneal macrophages. It suggested that intracellular GSH plays an important role in regulation of NO production and protection of host cells from cytotoxic attack induced by NO.
