Preliminary attempt to identify geoherbalism of Flos Lonicerae by sequence divergence of 5S-rRNA gene spacer region
- VernacularTitle:5S-rRNA基因间区序列变异用于金银花药材道地性研究初探
- Author:
Ping LI
;
Zhaohui CAI
;
Junbo XING
;
- Publication Type:Journal Article
- Keywords:
Flos Lonicerae;
geoherbalism;
sequence of 5S rRNA gene spacer region
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To study the formation of geoherbalism of Flos Lonicerae on the molecular level Methods Genomic DNAs from different populations of Lonicera japonica Thunb , outgroup Lonicera similis Hemsl , and Lonicera confusa DC were extracted, the 5S rRNA gene spacer region amplified, sequuenced, and analysed with Mega 1 02 Results The fragments of 5S rRNA gene spacer region in Lonicera L was about 210 bp with G+C content up to about 70% The sequences were different in various populations, and can be identified by sequencing The genetic distance between L confusa and L japonica was larger Conclusion The genuine distance between species was larger than that within species; the genetic distance among genuine crude drugs was smaller; the genetic distance among genuine and ungenuine crude drugs were bigger than that among genuine crude drugs
