Induction of rat cerebellar granular neuron apoptosis by 1-methyl-4-phenyl pyridinium cation
- VernacularTitle:1-甲基-4-苯基吡啶离子诱发大鼠小脑颗粒细胞凋亡
- Author:
Xiaoping PU
;
Xiaorong LI
;
Huinong LI
;
Changling LI
;
- Publication Type:Journal Article
- Keywords:
methyl 4 phenyl pyridinium cation (MPP +);
cerebellar granular neuron (CGN);
apoptosis
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To establish a rat cerebellar granular neuron (CGN) apoptosis model by 1 methy 4 phenyl pyridinium cation (MPP +) Methods Rat CGN were treated with MPP + and the resulting cell morphology examined by methyl green pyronine staining, agarose gel electrophorsis of DNA and flow cytometry Results MPP + at the concentration of 50 ? mol/L can induce CGNs apoptosis of the established model Conclusion The CGNs apoptosis model induced by MPP + can be used for the study on regulatory mechanism of cell apoptosis and the screening of antiparkinsonian drugs
