Determination of Berberiue hydrochloride and Palmatine hydrochloride in Wuji Pills by HPLC
- VernacularTitle:HPLC测定戊己丸中盐酸小檗碱和盐酸巴马汀的含量
- Author:
Peifen JIN
;
Huarong MIAO
;
Yaping QIAN
;
Bo CUI
;
- Publication Type:Journal Article
- Keywords:
HPLC;
Berberine hydrochloride;
Palmatine hydrochloride;
Wuji Pil
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the method of determination of the content of Berberine hydrochloride and Palmatine hydrochloride in Wuji Pills. Methods: Ultrasonic extraction, HPLC was used to determine Berberine hydrochloride and Palmatine hydrochloride in Wuji Pills on C 18 Column, using 0.033mol/L KH 2PO 4 CH 3CN(90∶40) as mobile phase, with detection at 265nm. Results: The linearity of this method was well. The average recoveries were 99.07% for Berberine hydrochloride and 98.59% for Palmatine hydrochloride. RSD were 1.3% and 1.7%, respectively. Conclusion: This method shows good separating degree and can be used for quality control of Wuji Pills.
