The adaptive cytoprotection of H_2O_2 preconditioning against oxidative-stress damage in PC12 cell
- VernacularTitle:H_2O_2预处理对PC12细胞氧化应激损伤的适应性细胞保护作用
- Author:
Xiaoqing TANG
;
Jianqiang FENG
;
Pingyang LI
;
Ruixian GUO
;
Meihong YE
;
- Publication Type:Journal Article
- Keywords:
H 2O 2;
preconditioning;
oxidative stress;
adaptive cytoprotection;
brain derived neurotrophic factor
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the adaptive cytoprotection of H 2O 2 preconditioning against oxidative stress damage in PC12 cell and the relationships between brain derived neurotrophic factor (BDNF) and the adaptive cytoprotection of H 2O 2 preconditioning. METHODS The viability of PC12 cells was evaluated by MTT assay. Apoptosis was detected by PI stain flow cytometer. Expression of BDNF was analyzed by immuo flow cytometer. RESULTS After H 2O 2 preconditioning, the survival of PC12 cells exposure to H 2O 2 (20～60 ?mol?L -1 ) was increased and the apoptosis of PC12 cells induced by H 2O 2 (20 or 30 ?mol?L -1 ) was inhibited and the expression of BDNF in PC12 cells was enhanced. CONCLUSION H 2O 2 preconditioning is protective against the damage of PC12 cells induced by H 2O 2 and its mechanisms may involve up modulation of the expression of BDNF.
