Effects of Scutellarin on glutathione peroxidase and thioredoxin reductase gene expression and activities in rats supplemented with upper limit Selenium
- VernacularTitle:灯盏花素对UL补硒大鼠谷胱甘肽过氧化物酶和硫氧还蛋白还原酶基因表达和活性的影响
- Author:
Abdella ALI
;
Lu GAN
;
Qiong LIU
;
Hong LIU
;
Huibi XU
;
- Publication Type:Journal Article
- Keywords:
selenium;
scutellarin;
glutathione peroxidase;
thioredoxin reductase;
gene expression;
enzyme activity
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effects of Scutellarin on glutathione peroxidase and thioredoxin reductase gene expression and activities in liver, kidney and testis tissues of rats supplemented with upper limit intake (UL) of Se. METHODS RT PCR and enzyme activities were used to determine GSH Px and TR mRNA levels and activities. RESULTS GSH Px and TR mRNA levels and activities were remarkably decreased in livers of rats supplemented with UL Se. Scutellarin significantly increased GSH Px and TR mRNA levels and activities in livers of rats supplemented with UL Se. No significant effects were found in rats treated with only Scutellarin as compared with the control. There were also no significant effects of UL Se on GSH Px and TR mRNA levels and activities in rat kidney and testis. CONCLUSION Scutellarin effectively antagonized the decrease of GSH Px and TR mRNA levels and activities in rat livers due to supplementation with UL. The effect of UL on GSH Px and TR mRNA levels shows tissue specificity.
