Protective action and of onychin aganist growth inhibition of endothelial cell injured by oxidation and its mechanism
- VernacularTitle:金粉蕨素拮抗氧化损伤所抑制的内皮细胞增殖的作用及其机制
- Author:
Yu GUO
;
Bingyang ZHU
;
Fengxiang YAN
;
Duanfang LIAO
;
- Publication Type:Journal Article
- Keywords:
onychin;
endothelial cell;
endothelial nitric oxide synthase(eNOS), extracellular signal regulated kinase 1 and 2(ERK1/2)
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the protective action of onychin aganist the growth inhibition of endothelial cell injured by menadione and its mechanism. METHODS The injured model was established by endothelial cell treated with menadione.Protective effect of onychin aganist growth inhibition of injured endothelial cell was determined by MTT assay and cell counting method; NO concentration in the medium was determined by nitrate reductase assay; eNOS and phosph ERK1/2 protein levels were determined by Western blot. RESULT Onychin significantly decreased the growth inhibitory rate of injured endothelial cells,increased NO concentration in the medium and eNOS activity and up regulated phosph ERK1/2 expressing. CONCLUSION Onychin has protective action and against the growth inhibition of endothelial cell injured by menadione may be via NO and ERK1/2 signal pathway.
