Effects of propofol on plasma membrane fluidity in PC12 cells and liposome
- VernacularTitle:异丙酚对PC12细胞和脂质体膜流动性的影响
- Author:
Jinrong ZHANG
;
Caizhu LIN
;
Xixin YANG
;
Kairong LU
;
Guoqing SHENG
;
Tijun DAI
;
- Publication Type:Journal Article
- Keywords:
pharmacology;
propofol;
cells,PC12;
liposome;
membrane fluidity
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of propofol on plasma membrane fluidity in PC12 cells and liposome,and its relevant mechanism. METHODS: Fluorescence depolarization method was used to measure values of fluorescence anisotropy, fluorescence polarization as well as microviscosity in PC12 cells and microviscosity in liposome continuously for 30 min. RESULTS: Propofol induced a significant decrease of fluorescence anisotropy, fluorescence polarization as well as microviscosity in PC12 cells, particularly in the first 5 min. After 5 min, the values of anisotropy were remained lower levels. Although propofol at concentration of 1 mg?L -1 had no effects on microviscosity in liposome, porpofol at concentration of 10 mg?L -1 and 100 mg?L -1 significantly decreased microviscosity in liposome. CONCLUTION: Propofol can significantly increase membrane fluidity in PC12 cells and liposome in a concertration dependent manner, and the anesthetic effect of propofol may be resulted from changes of membrane fluidity and structure of neurocyte.
