Establishment of rat cerebral hemorrhage model by type Ⅳcollagenase injection
- VernacularTitle:采用Ⅳ型胶原酶构建大鼠脑出血模型
- Author:
Yanling ZHANG
;
Kangning CHEN
;
Shuqin SHAO
;
Ying WANG
;
- Publication Type:Journal Article
- Keywords:
collagenase;
cerebral hemorrhage;
animal model
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a cerebral hemorrhage model in rats by local injection of type Ⅳcollagenase, and explore the factors affecting the speed and volume of hematoma formation Methods A total of 150 healthy male Wistar rats weighing from 200 to 250 g were randomly divided into 3 groups, control, collagenase, and collagenase/heparin groups Animals in those groups received injection of saline, collagenase (0 2 U/?l), and collagenase (same dose) and heparin (2 U/?l) with different volume respectively at the caudate nucleus The volume of the hematoma in the rats ( n =6 at each time point) was observed 6, 12, and 24 h, and 3, 7d after the injection Results Permeation of blood was found in 12 h after injection in control group Hematoma about 3 mm in diameter was found in 3 d after injection in collagenase group, while in collagenase/heparin group, it was found in 24 h Conclusion Cerebral hemorrhage model established by local injection of collagenase/heparin in saline solution is ideal and reliable, and the size of hematoma is in correlation with the volume of solutions injected into the brain
