Effect of NADH on the damage of L02 cells undergoing X-ray irradiation
- VernacularTitle:ADH对抗X射线照射后L02肝细胞损伤作用
- Author:
Faquan LIU
;
Jiren ZHANG
;
Xiaoping XU
;
- Publication Type:Journal Article
- Keywords:
NADH;
antiradiation;
ROS
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study effects of antioxidant NADH on damage of irradiated normal cell lines. METHODS L02 liver cells were cultured in RPMI 1640, exposed to X ray irradiation, and continued to culture in the presence or absence of NADH for 24 h. The cellular viability was determined by routine MTT method. Using fluorescence probe and confocal microscope, the level of cellular H 2O 2 was detected. Positive rate of bcl 2 and Bax protein expression in the L02 cells were analyzed by flow cytometry. RESULT NADH can not only antagonized growth inhibition of X ray irradiated L02 cells, decreased cellular H 2O 2 production, but also significantly increased positive rate of L02 cells expressed bcl 2 protein and decrease positive rate of L02 cells expressed Bax proteins ( P
