Effects of tRA on activity and content of uncoupling protein in rat brown adipose tissue mitochondria
- VernacularTitle:全反式维甲酸对大鼠棕色脂肪线粒体上解偶联蛋白活性和含量的影响
- Author:
Zhenchun MIAO
;
Dajin ZOU
;
Xuemin WANG
;
- Publication Type:Journal Article
- Keywords:
tretinoin;
brown rat;
metabolism;
uncoupling protein;
purine nucleotides
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate effects of tRA on activity and content of UCP in rat brown adipose tissue mitochondria. Methods: The rats were divided into tRA treatment and control groups using 3H GTP binded UCP in mitochondria. Radioactivity was measured by liquid aglimmer instrument,the dissociation constant( K d)and maximal binding( B max )was determined from Scathard plot. Results: In tRA treatment rat, B max increased by 1.48 fold, K d remained unchanged, rat body weight loss and brown fat weight decreased, white fat became rosiness. Conclusion: tRA can increase concentration of UCP in brown adipose tissue mitochondria, but do not change its activity,it also can increase fat consumption,produce UCP from white adipose tissue. [
