Construction of DNA vaccine pcD-flaB against pathogenic leptospira infection and its immune mechanism
- VernacularTitle:钩端螺旋体DNA疫苗pcD-flaB的构建及其免疫机制的初步研究
- Author:
Zhihua KOU
;
Pingwu ZHANG
;
Shuhan SUN
;
- Publication Type:Journal Article
- Keywords:
leptospira;
DNA;
vaccines;
macrophage;
T
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the immume mechanism and protective effect of DNA vaccine pcD flaB against pathogenic leptospira infection. Methods: DNA vaccine pcD flaB was constructed by inserting flaB into the eucaryotic expressing plasmid pcDNA3. After guinea pigs were infected with leptospira, the protective rate was observed and specific anti leptospira antibody IgG were tested by ELISA. TNF activity was tested by cell proliferation. Results:The protective rate against leptospira infection was 100%. The specific antibody IgG generated peaked at the 6th week. Activity of TNF released by macrophage of guinea pigs given DNA vaccine was higher than that not given vaccine. Conclusion:DNA vaccine pcD flaB can protect guinea pigs from leptospira challenge infection by inducing humoral immune response and increasing TNF activity.
