Short-term Efficacy of Topical Immunosuppressive Agents on the Survival of Cultivated Allo-Conjunctival Equivalents.
10.3341/kjo.2008.22.2.123
- Author:
Young Joo SHIN
1
;
Mee Kum KIM
;
Joo Youn OH
;
Won Ryang WEE
;
Jin Hak LEE
;
Jung Hwa KO
;
Hyun Ju LEE
;
Jae Lim LEE
;
Byung Moo MIN
;
Young Suk SOHN
Author Information
1. Department of Ophthalmology, Dongguk University College of Medicine, Goyang, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cultivated allo-conjunctival equivalents;
Steroid;
Topical immunosuppressants;
Rapamycin
- MeSH:
Administration, Topical;
Animals;
Cell Count;
*Cell Transplantation;
Cells, Cultured;
Conjunctiva/*cytology;
Cyclosporine/pharmacology;
Epithelial Cells/metabolism/*transplantation;
Female;
Fluorescent Antibody Technique, Indirect;
Graft Survival/*drug effects;
Immunosuppressive Agents/*pharmacology;
Keratin-4/metabolism;
Keratin-7/metabolism;
Male;
Organic Chemicals/metabolism;
Prednisone/pharmacology;
Rabbits;
Sirolimus/pharmacology;
Transplantation, Homologous
- From:Korean Journal of Ophthalmology
2008;22(2):123-129
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To investigate the short-term efficacy of topical immunosuppressive agents on the survival of cultivated allo-conjunctival equivalents. METHODS: Twenty-five eyes of New Zealand white rabbits were included. Temporal conjunctivae were trephined to a diameter of 7.5 mm, and then cultured allo-conjunctival epithelial cells on amniotic membrane were transplanted onto them. Various immunosuppressants including steroid, cyclosporine, and rapamycin were applied topically four times a day for a week. Epithelial defects and graft edema were graded daily. Numbers of inflammatory cells were measured in H&E. PKH26 and cytokeratin 4 and 7 were immunostained. RESULTS: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes. In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7. Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes. CONCLUSIONS: Topical steroid or rapamycin can help to suppress acute inflammation and enhance the acute survival of transplanted conjunctival cells.
