Inhibitory effect on human stomach adenocarcinoma BGC-823 cell line of cyclin D_1 antisense oligodeoxynucleotide
- VernacularTitle:细胞周期素D_1反义寡核苷酸对胃癌细胞株BGC-823的作用
- Author:
Xueqin WANG
;
Linxi YANG
;
Yuewu HAN
;
- Publication Type:Journal Article
- Keywords:
stomach neoplasm;
stomach adenocarcinoma;
cyclin D 1;
oligodexynucleotide
- From:
China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To investigate the effect of in vitro antisense oligodeoxynucleotide of cyclin D 1 on the cyclin D 1 gene expression and cell proliferation of human stomach adenocarcinoma cell BGC 823 cell line.Methods:Phosphorothioate cyclin D 1 ASODN and random oligodeoxynucleotide (RODN) were synthesized and transfected into BGC 823 Cells. Their effects on cell proliferation were examined by MTT method,RT PCR method,immunohistochemical study.Results:Cyclin D 1 ASODN could significantly inhibit the growth of BGC 823 Cell lines.The RODN showed no such effect.The inhibition peaked at 48 hour after transfection by MTT method and was dose dependent.ASODN could downregulate the expression levels of cyclin D 1 mRNA and protein by RT PCR method and immunohistochemical study respectively.Conclusions:The data suggested that ASODN could specifically inhibit the expression of cyclin D 1 mRNA and protein and regulate cell cycle and cell proliferation of BGC 823 cells. [
