Recombinant human interleukin-10 inhibits the vascular smooth muscle cell proliferation in rat stimulated by angiotensin II
- VernacularTitle:人白细胞介素10对血管紧张素II诱导的大鼠血管平滑肌细胞增殖的作用
- Author:
Ping OUYANG
;
Lisheng PENG
;
Hong YANG
;
Wenlie PENG
;
Anlong XU
;
- Publication Type:Journal Article
- Keywords:
Muscle,smooth ,vascular;
Interleukin 10;
Angiotensin II;
Mitogen activated protein kinases
- From:
Chinese Journal of Geriatrics
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Vessel injury provokes the release of angiotensin II that influences the proliferation and migration of vascular smooth muscle cells (VSMCs). T lymphocytes produced, interleukin 10 is of immunoregulatory function. The purpose of this study is to determine the effects of recombinant human interleukin 10 (rhIL 10) on the proliferation of isolated rat vascular smooth muscle cell and the activity of p44/p42 mitogen activated protein kinase (MAPK) promoted by angiotensin II. Methods Rat aortic VSMCs were cultured and treated with rhIL 10 or angiotensin II, and then co treated with rhIL 10 and angiotensin II. The proliferation was quantified by colormetric assay. The p44/p42 MAPK activity was evaluated by the immunobloting technique using anti p44/p42 phospho MAPK antibody. Results Compared with control group, angiotensin II stimulated significantly VSMCs proliferation(1 311?0 201 vs 0 781?0 236, P 0 05), but significantly inhibited VSMCs proliferation induced by angiotensin II at a dose of 1,10,100 ng/ml (0 984?0 172 , 0 932?0 134 , 0 784?0 097 vs 1 311?0 201, P
