The expression of hepatocyte growth factor in experimental cyclosporin A nephropathy in rats
- VernacularTitle:肝细胞生长因子在环孢素A肾病大鼠肾组织中的表达意义
- Author:
Guohua ZHANG
;
Xun ZHANG
;
Fanfan HOU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Hepatocyte growth factor;
Cyclosporine;
Kidney tubules;
Rats
- From:
Chinese Journal of Urology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of hepatocyte growth factor (HGF) in cyclosporin A nephropathy model of SD rats. Methods Twenty eight rats were fed on low salt diet for 7 days,then they were randomly divided into four groups:controls(n=7),CsA treatment (n=7),CsA+verapamil (n=7),and CsA+enalapril (n=7).All the rats except for controls received a daily subcutaneous injection of CsA (15 mg?kg -1 ?d -1 ) for 4 weeks.Renal tissue angiotensin Ⅱ level was measured by radioimmunoassay.The expression of HGF mRNA was examined by Northern blot and in situ hybridization. Results Renal tissue angiotensin Ⅱ level were significantly elevated in CsA treated rats when compared to that in control rats [(29.8?6.0) vs (8.7?1.7)ng/g, P 0.05 ).Renal tissue HGF mRNA expression was negatively correlated with tubular injury degree ( P
