High level expression of human truncated insulin like growth factor 1 in E.coli
- VernacularTitle:重组人截短型胰岛素样生长因子1在大肠杆菌中的高效表达
- Author:
Ying LI
;
Hongye DENG
;
Chunhui DI
;
Al ET
- Publication Type:Journal Article
- Keywords:
Truncated insulin like growth factor 1 E.coli High level expression
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an efficient expression system for human truncated insulin like growth factor 1 〔Des(1 3)IGF1〕as fusion protein in Escherichia coli(E.coli). Methods: The cDNA of Des(1 3)IGF1 was cloned into an fusion protein expression plasmid, pMTY4, using gene recombinant technique. The protein was purified by ion exchange chromatography and identified by SDS polyacrylamide gel electrophoresis, radioimmunoassay(RIA), N terminal amino acid sequence and biological activity. Results: A prokaryotic expression vector was constructed and the fusion protein containing MS2 polymerase fragment, thrombin recognition site and human Des(1 3)IGF1 was expressed in E.coli at high level. It was showed that the purified recombinant Des(1 3)IGF1 released from the fusion protein after digestion with thrombin was identical to the native Des(1 3)IGF1.Conclusion:This is an effective method for obtaining human recombinant Des(1 3)IGF1 and it is very important for further study of Des(1 3)IGF1.
