Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera：Cimicidae)
10.1016/j.apjtb.2015.04.012
- Author:
Zahran Zulaikha
;
Ismail Azliza Nor
;
Rahman Abdul Wardah
;
Dieng Hamady
;
Satho Tomomitsu
- Publication Type:Journal Article
- Keywords:
Cimex hemipterus;
Aspergillus;
Trichoderma;
β-tubulin;
Internal transcribed spacer;
Morphological characteristics
- From:Asian Pacific Journal of Tropical Biomedicine
2015;(9):684-690
- CountryChina
- Language:Chinese
-
Abstract:
To investigate some morphological and molecular characteristics of fungal parasites isolated from wild tropical bed bug, Cimex hemipterus. Methods: A series of culture methods were used to obtain fungal isolates from field-collected bed bugs. Characteristics of the isolates such as colony appearance, mycelial texture and pigmentation were studied to explore their morphology. Isolates were also subjected to a PCR-based genotyping test. Results: There were noticeable differences in morphological characteristics among the four isolates. Conidial areas of one isolate were dark green, whereas those of the remaining colonies were olive-green, black or dark brown. Conidia of the dark green isolate were globose, while those of olive-green, black and dark brown isolates were globose to subglobose, globose to spherical and globose to subglobose/finely roughened, respectively. These morphological specificities and the molecular analyses showed that the fungal internal transcribed spacer ribosomal region and β-tubulin gene sequences of the isolates shared clade with Trichoderma and Aspergillus sequences. Conclusions: Overall, the new discovery of common pathogens in agricultural field developed in live bed bugs storage tank may initiate the use of biological agents in later years.
