Ethanol extracts of Scutellaria baicalensis protect against lipopolysaccharide-induced acute liver injury in mice
10.1016/j.apjtb.2015.07.007
- Author:
Thanh Nguyen Hai
;
Le Anh Tuan
;
Huu Nguyen Tung
;
Duc Vu Loi
;
Kim Dang Thu
;
Thanh Bui Tung
- Publication Type:Journal Article
- Keywords:
Scutellaria baicalensis;
Lipopolysaccharide;
Liver injury;
Cytokine;
Nuclear factor-κB
- From:Asian Pacific Journal of Tropical Biomedicine
2015;(9):733-738
- CountryChina
- Language:Chinese
-
Abstract:
To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis ) against lipopolysaccharide (LPS)-induced liver injury. Methods: Dried roots of S. baicalensis were extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS (5 mg/kg of body weight, intraperitoneal injection). Both protein and mRNA levels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB protein levels in liver tissues were analyzed by western blotting. Results: Liver injury induced by LPS significantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensis prevented all of these observed changes associated with LPS-induced injury in liver mice. Conclusions: Our study showed that S. baicalensis is potentially protective against LPS-induced liver injury in mice.
