Role of HIF-1α in reduction of apoptosis in cortical neurons of rats by sevoflurane preconditioning: the relationship with Bid, Bim and Puma
10.3760/cma.j.issn.0254-1416.2015.04.010
- VernacularTitle:HIF-1α在七氟醚预处理减轻大鼠皮质神经元凋亡中的作用:与Bid、Bim和Puma的关系
- Author:
Wenbo SUN
;
Limin ZHANG
;
Li'na KANG
;
Chunling WU
;
Dongdong HUANG
;
Xiuwei SUN
;
- Publication Type:Journal Article
- Keywords:
Hypoxia-inducible factor 1,alpha subunit;
Apoptosis regulatory proteins;
Anesthetics,inhalation;
Ischemic preconditioning;
Cell hypoxia;
Oxygen;
Neurons;
Apoptosis
- From:
Chinese Journal of Anesthesiology
2015;35(4):430-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of hypoxia inducible factor-1α (HIF-1α) in reduction of apoptosis in cortical neurons of rats by sevoflurane preconditioning.Methods Primary cortical neurons obtained from neonatal Sprague-Dawley rats were seeded in 6-well plates (2 ml/well),and randomly divided into 4 groups (n =15 each) using a random number table:control group (C group),anoxiareoxygenation (A/R) group,sevoflurane preconditioning group (SP group) and HIF-1α inhibitor 2-methoxyestradiol group (H group).The neurons were subjected to O2-glucose deprivation for 90 min followed by restoration of O2-glucose supply for 24 h.In group SP,the neurons were exposed to 2.0％ sevoflurane for 2 h followed by 5 min washout for 3 times,and then sevoflurane preconditioning was performed immediately.In group H,sevoflurane preconditioning was performed at 72 h of incubation with 5 μmol/L 2-methoxyestradiol.The apoptosis in neurons was assessed using Annexin V-FITC/PI assay,and apoptosis rate was calculated.The expression of Bid,Bim,Puma and activated caspase-3 in neurons was detected by Western blot.Results Compared with group C,apoptosis rate was significantly increased,and the expression of Bid,Bim,Puma and activated caspase-3 was up-regulated in group A/R.Compared with group A/R,apoptosis rate was significantly decreased,and the expression of Bid,Bim,Puma and activated caspase-3 was down-regulated in group SP.Compared with group SP,apoptosis rate was significantly increased,and the expression of Bid,Bim,Puma and activated caspase-3 was up-regulated in group H.Conclusion HIF-1α mediates reduction of apoptosis in rat neurons by sevoflurane preconditioning,and down-regulated expression of Bid,Bim,and Puma is involved in the mechanism.
