Simultaneous Determination of Puerarin and Baicalin in Xiao’ er Shuangjie Zhixie Granules by HPLC
- VernacularTitle:HPLC法同时测定小儿双解止泻颗粒中葛根素和黄芩苷的含量
- Author:
Shengna ZHANG
;
Suxiang WU
;
Haina XUE
;
Qian JIANG
- Publication Type:Journal Article
- Keywords:
Xiao’ er Shuangjie Zhixie granules;
Pueratin;
Baicalin;
HPLC
- From:
China Pharmacist
2015;(5):863-864,865
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of puerarin and balcalin in Xiao’ er Shuangjie Zhixie granules by HPLC. Methods:An Agilent Zorbax SB-C18 column (250 mm × 4. 6 mm,5 μm) was used. The mobile phase A was 0. 1% phos-phoric acid solution and the mobile phase B was methanol with gradient elution. The detection wavelength was 265 nm,the flow rate was 1. 0 ml·min-1 ,the column temperature was 30℃ and the sample size was 20 μl. Results: The linear range of pueratin was 0. 121-1. 942 μg (r=0. 999 5)and that of balcalin was 0. 230-3. 686 μg(r=0. 999 5). The average recovery was 98. 10% and 98. 20%with RSD of 1. 34% and 1. 11%(n=6), respectively. Conclusion:The method is convenient and accurate, which can be used in the de-termination of pueraln and balcalin in Xiao’ er Shuangjie Zhixie granules.
