Content Determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Shuxuening Capsules by HPLC
- VernacularTitle:HPLC法同时测定舒血灵胶囊中三七皂苷R1、人参皂苷Rg1及人参皂苷Rb1的含量
- Author:
Daohai CHENG
;
Jie WANG
;
Hua LU
;
Zhenguang HUANG
- Publication Type:Journal Article
- Keywords:
Shuxuening capsules;
Notoginsenoside R1;
Ginsenoside Rg1;
Ginsenoside Rb1;
HPLC
- From:
China Pharmacist
2015;(11):1978-1980
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the content of notoginsenoside R1 , ginsenoside Rg1 and Rb1 in Shuxuening capsules. Meth-ods:The three constituents were determined on a Hypersil ODS-2 C18 column (250 mm × 4. 6 mm, 5 μm) with gradient elution using acetonitrile (A) -aqueous solution (B) (0-8 min, 20%A→20%A, 8-40 min, 20%A→30%A, 40-60 min, 30%A→45%A) at the detection wavelength of 203 nm with a flow rate of 1. 0 ml·min-1 . The column temperature was 25℃ and the injection volume was 20μl. Results:The calibration curve showed good linearity within the concentration range of 0. 05-0. 50 mg·ml-1 for notogisenoside R1 , and 0.20-2.00 mg·ml-1 for ginsenoside Rg1 and Rb1(r =0.999 9). The average relative recovery was 98.79%, 98.42% and 98. 89% for each constituent(RSD=0. 85%, 0. 97% and 0. 74%, respectively, n=6). The intra-day RSD was 0. 49%, 0. 20% and 0. 39%, and the inter-day RSD was 0. 75%, 0. 56% and 0. 51%, respectively. The RSDs of stability test and repeatability test were less than 1%. Conclusion:The method is simple with good accuracy and repeatability, which can be used for the determination of no-toginsenoside R1 , ginsenoside Rg1 and Rb1 in Shuxuening capsules.
