Determination of Lidocaine Hydrochloride in Buccal Adhesive Tablets Containing Carbomer by HPLC
- VernacularTitle:HPLC法测定卡波姆类口腔黏附片中盐酸利多卡因的含量
- Author:
Xudong FU
;
Chen CHEN
;
Yinke LI
;
Shanshan WANG
;
Xiangyang XIE
- Publication Type:Journal Article
- Keywords:
Lidocaine hydrochloride;
HPLC;
Carbomer;
Buccal adhesive tablets
- From:
China Pharmacist
2015;(4):695-697
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method to determine the content of lidocaine hydrochloride in buccal adhesive tablets contai-ning carbomer as an excipient by HPLC. Methods:Calcium chloride was used to precipitate carbomer in buccal adhesive tablets. The HPLC analysis was performed on a Welch C18 column(250 mm × 4. 6 mm,5 μm)with the column temperature of 40℃. The mobile phase was the mixture of 0. 05% sodium acetate solution(added 50 ml acetic acid into 930 ml distilled water,adjusting pH to 3. 40 with 1 ml·min-1 sodium hydroxide solution)and acetonitrile(70:30). The flow rate was 1. 0 ml·min-1 ,the detection wavelength was at 254 nm and the injection volume was 20 μl. Results:Within the range of 0. 10-2. 00 mg·ml-1 ,there was a good linear rela-tionship between the concentration and the peak area of lidocaine hydrochloride(r=0. 999 9). The average recovery was 100. 2%( RSD=1. 4%,n=9). Conclusion:The established method is simple,accurate and reproducible,which can be applied to determine the content of lidocaine hydrochloride in buccal adhesive tablets.
