Effect of Scutellarin on the Expression and Activity of P-gp in Caco-2 Cells
- VernacularTitle:野黄芩苷对Caco-2细胞中P-gp蛋白表达及活性的影响
- Author:
Rui LIU
;
Jing JIN
;
Xiaoli GOU
;
Ziyi ZHOU
;
Yefeng CAI
;
Min HUANG
- Publication Type:Journal Article
- Keywords:
Scutellarin;
P-gp;
Protein expression;
Rhodamine-123;
Caco-2
- From:
China Pharmacist
2015;(4):529-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of scutellarin on P-gp protein expression and activity in Caco-2 cells. Methods:Scutellarin(25,50 and 100 μmol·L-1 )was incubated with Caco-2 cells respectively for 24 h,48 h and 72 h. The expression of P-gp was determined by western blot assay and the activity of P-gp was determined by Rhodamine-123 assay. Results:P-gp protein ex-pression levels were significantly increased by scutelarin. After the incubation for 24 h with scutellarin,P-gp protein expression was up-regulated 2. 34-,2. 65-and 2. 00-fold in Caco-2 cells. After the incubation with scutellarin for 48 h,P-gp protein expression was up-regulated 2. 70-,4. 66-and 3. 13-fold. After the incubation with scutellarin for 72 h,P-gp protein expression was up-regulated 2. 82-, 2. 62-and 1. 84-fold. The intracellular accumulation of rhodamine-123 was significantly decreased by scutellarin,indicating that the ef-flux transport activity of P-gp was increased by scutellarin in Caco-2 cells. Conclusion:Scutellarin can significantly up-regulate P-gp protein expression and increase the efflux transport activity of P-gp in Caco-2 cells.
