Simultaneous Determination of 4 Components in Fule Granules by HPLC
10.6039/j.issn.1001-0408.2017.33.34
- VernacularTitle:HPLC法同时测定妇乐颗粒中4种成分的含量
- Author:
Xiaoxia LUO
1
;
Caicai ZENG
;
Longkai QI
Author Information
1. 广东省中医院药学部
- Keywords:
Fule granules;
HPLC;
Gallic acid;
Chlorogenic acid;
Paeoniflorin;
Paeonol;
Content
- From:
China Pharmacy
2017;28(33):4732-4734
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of gallic acid,chlorogenic acid,paeoniflorin and paeonol in Fule granules. METHODS:HPLC method was adopted. The determination was performed on Ultimate XB-C18 col-umn with mobile phase consisted of acetonitrile-0.05%phosphoric acid(gradient elution)at the flow rate of 1.0 mL/min. The detec-tion wavelengths were 270 nm(gallic acid,paeonol),325 nm(chlorogenic acid)and 230 nm(paeoniflorin). The column tempera-ture was 30 ℃,and sample size was 10 μL. RESULTS:The linear ranges of gallic acid,chlorogenic acid,paeoniflorin and pae-onol were 0.0762-1.524 μg(r=0.9997),0.0376-0.751 μg(r=0.9999),0.0303-0.606 μg(r=0.9997),0.0206-0.412 μg(r=0.9998),respectively. The limits of quantification were 0.353,0.276,0.421,0.540 μg/mL,and the limits of detection were 0.121,0.104,0.148,0.186μg/mL. RSDs of precision,stability and reproducibility tests were all no more than 2.04%. The recover-ies were 95.24%-100.47%(RSD=1.59%,n=9),99.49%-103.70%(RSD=2.27%,n=9),96.27%-101.09%(RSD=1.94%,n=9),95.05%-98.89%(RSD=1.22%,n=9), respectively. CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the simultaneous determination of gallic acid,chlorogenic acid,paeoniflorin and paeonol in Fule granules.
